Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Characterization of parallel and antiparallel G-tetraplex structures by vibrational spectroscopy.

M Romero Guzmán1, J Liquier, S K Brahmachari

  • 1Laboratoire BioMoCeTi, UMR CNRS 7033, Université Paris 13, 74, rue Marcel Cachin, F93017 Bobigny Cedex, France.

Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
|December 31, 2005
PubMed
Summary

FTIR spectroscopy reveals distinct spectral markers for guanine tetrad formation in G-rich oligonucleotides. A new marker, the guanine carbonyl stretching vibration, differentiates parallel and antiparallel tetraplex structures.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Targeting of Pu.Py Duplexes by GA and GT Rich Oligonucleotides on Microchip and in Solution.

Journal of biomolecular structure & dynamics·2012
Same author

Towards new business models for R&D for novel antibiotics.

Drug resistance updates : reviews and commentaries in antimicrobial and anticancer chemotherapy·2011
Same author

[A big purple leg].

La Revue de medecine interne·2010
Same author

Campylobacter fetus bloodstream infection: risk factors and clinical features.

European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology·2007
Same author

Mechanism of copper mediated triple helix formation at neutral pH in Drosophila satellite repeats.

Biophysical journal·2007
Same author

Evidence of linkage and association on 18p11.2 for psychosis.

American journal of medical genetics. Part B, Neuropsychiatric genetics : the official publication of the International Society of Psychiatric Genetics·2006

Area of Science:

  • Biophysical Chemistry
  • Spectroscopy
  • Oligonucleotide Structure

Background:

  • G-rich oligonucleotides can form stable tetraplex structures, crucial in biological processes.
  • Understanding the structural nuances of these tetraplexes (parallel vs. antiparallel) is vital for their functional characterization.

Purpose of the Study:

  • To identify characteristic FTIR spectral markers for guanine tetrad formation.
  • To develop a novel FTIR-based marker to distinguish between parallel and antiparallel tetraplexes.
  • To investigate tetraplex reorganization in specific telomeric sequences.

Main Methods:

  • Fourier-transform infrared (FTIR) spectroscopy was employed to analyze G-rich oligonucleotides.
  • Characteristic absorption bands associated with guanine tetrads were identified.

Related Experiment Videos

  • The position of the guanine C6O6 carbonyl stretching vibration was analyzed to differentiate tetraplex orientations.

    • Main Results:

    • Distinct FTIR markers for guanine tetrad formation were established.
    • A novel marker, the C6O6 guanine carbonyl stretching vibration, successfully discriminated between parallel (1693 cm⁻¹) and antiparallel (1682 cm⁻¹) tetraplexes.
    • FTIR evidenced antiparallel-parallel tetraplex reorganization in Oxytricha nova and human telomeric sequences upon ion exchange.

    Conclusions:

    • FTIR spectroscopy provides valuable insights into guanine tetraplex formation and structure.
    • The C6O6 carbonyl stretching vibration serves as a reliable marker for distinguishing parallel and antiparallel tetraplexes.
    • Tetraplex structures are dynamic and can undergo reorganization, influenced by ionic environments.