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Updated: Aug 13, 2026

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Single Droplet Digital Polymerase Chain Reaction for Comprehensive and Simultaneous Detection of Mutations in Hotspot Regions
Published on: September 25, 2018
Detection of new single nucleotide polymorphisms by means of real time PCR
M Reuter1, Y Küpper, A Schmitz
1Center of Psychobiology and Behavioural Medicine, Justus-Liebig-University of Giessen, Otto-Behaghel-Str. 10F, D-35394 Giessen, Germany. martin.reuter@psychol.uni-giessen.de
Journal of Genetics
|December 31, 2005
Abstract
No abstract available in PubMed .
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Real Time RT-PCR
Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...
The real-time quantification of the number of amplified products is...
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