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Detection of new single nucleotide polymorphisms by means of real time PCR

M Reuter1, Y Küpper, A Schmitz

  • 1Center of Psychobiology and Behavioural Medicine, Justus-Liebig-University of Giessen, Otto-Behaghel-Str. 10F, D-35394 Giessen, Germany. martin.reuter@psychol.uni-giessen.de

Journal of Genetics
|December 31, 2005
PubMed
Abstract

No abstract available in PubMed .

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Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
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