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Diminished leukocyte-endothelium interaction in tumor microvessels
1Department of Radiation Oncology, Duke University Medical Center, Durham, North Carolina 27710.
Cancer Research
|August 1, 1992
Summary
Leukocyte-endothelium interactions are reduced in tumor blood vessels compared to normal ones. This suggests impaired adhesion molecule expression on tumor endothelial cells, impacting immune cell trafficking.
Area of Science:
- Oncology
- Immunology
- Vascular Biology
Background:
- Leukocyte-endothelium interactions, including rolling and adherence, are crucial for immune responses.
- These interactions are vital for leukocyte extravasation into tissues.
Purpose of the Study:
- To investigate leukocyte-endothelium interactions in a rat tumor microcirculation model.
- To compare these interactions in tumor versus normal microvessels under basal and inflammatory conditions.
Main Methods:
- Utilized a rat mammary adenocarcinoma skinfold window chamber model.
- Quantified leukocyte rolling flux and adherence density in tumor and normal microvessels.
- Measured microvessel blood flow shear rate.
Main Results:
- Leukocyte-endothelium interaction was significantly reduced in tumor microvessels.
- This reduction occurred under both control and inflammation-induced conditions (using N-formylmethionylleucylphenylalanine, lipopolysaccharide, and tumor necrosis factor alpha).
- Observed differences in shear rate did not explain the reduced leukocyte-endothelium interaction.
Conclusions:
- Tumor microvessels exhibit diminished leukocyte-endothelium interactions.
- This impairment is not solely due to altered shear rates.
- Suggests potential improper expression of adhesion molecules on tumor endothelial cells, affecting immune cell trafficking into tumors.