Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Solid tumors "melt" from the inside after successful CD8 T cell attack.

Ulrike Blohm1, Dan Potthoff, Albert J van der Kogel

  • 1Institute of Medical Microbiology and Hygiene, Department of Immunology, University of Freiburg, Germany.

European Journal of Immunology
|December 31, 2005
PubMed
Summary

Adoptive transfer of tumor-specific CD8 T cells can shrink tumors by attacking vasculature. This immunotherapy approach may enhance chemotherapy or radiation effectiveness by reducing tumor hypoxia.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

How the wild things are: comparative T cell phenotyping and memory T cell identification in wild boar and domestic pigs.

Frontiers in immunology·2026
Same author

CD8 T Cell Sensing of Type I Interferon Impacts Anergy.

European journal of immunology·2026
Same author

Increased Constitutive Interferon-β Levels and Altered CD4 T Cell Homeostasis Induced by Expression of a Viral Glycoprotein.

European journal of immunology·2026
Same author

Host genetics, lung T-cell immunity, and laying activity determine the disease outcome in avian influenza virus-infected chickens.

Veterinary research·2026
Same author

Baseline immune profiles of local chicken breeds: linking biodiversity, animal health, and vaccination response.

Poultry science·2025
Same author

Virulent African swine fever virus infection of porcine monocytes causes SLA I subversion due to loss of proper ER structure/function.

Journal of immunology (Baltimore, Md. : 1950)·2025

Area of Science:

  • Immunology
  • Cancer Biology
  • Translational Oncology

Background:

  • Adoptive T cell therapy is a promising cancer immunotherapy.
  • Understanding CD8 T cell interactions within tumors is crucial for optimizing treatment.

Purpose of the Study:

  • To visualize and characterize the in vivo anti-tumor response of adoptively transferred CD8 T cells.
  • To investigate the impact of T cell infiltration on tumor microenvironment and vascularization.

Main Methods:

  • Utilized P14 TCR-transgenic mice and immunohistology to track CD8 T cells specific for the GP33 antigen.
  • Adoptively transferred P14 T cells into tumor-bearing hosts with GP33-expressing tumors (3LL-A9 and MCA102).
  • Analyzed tumor sections for T cell infiltration, granulocyte accumulation, and vascular integrity (CD31 expression).

Related Experiment Videos

Main Results:

  • Adoptively transferred P14 T cells induced regression of established GP33-expressing tumors.
  • Tumor vasculature destruction and granulocyte infiltration were observed within the tumor mass.
  • P14 T cells localized to the tumor rim, while central areas showed granulocyte infiltration and lacked vasculature.
  • Treated tumors exhibited increased vascular density and reduced hypoxia in the rim zone compared to controls.

Conclusions:

  • Adoptive transfer of tumor-specific CD8 T cells effectively mediates tumor regression.
  • The anti-tumor effects involve T cell-mediated vascular damage and altered tumor microenvironment.
  • Reduced tumor hypoxia suggests potential synergy with conventional therapies like radiation and chemotherapy.