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Published on: September 10, 2016
Statistical screening of medium components by Plackett-Burman design for lactic acid production by Lactobacillus sp.
Kishor Chauhan1, Ujjval Trivedi, Kamlesh C Patel
1Department of Biosciences, Sardar Patel University, Vallabh Vidyanagar 380 120, Gujarat, India.
Abstract:
Statistical screening of media components for production of lactic acid by Lactobacillus sp. KCP01 using date juice as a sugar source was carried out by Plackett-Burman design. Date juice at 5% sugar concentration when used alone showed 2.6 g/l of lactic acid production. Increase in lactic acid production (15.1 g/l) was observed with supplementation of salts and organic nitrogen sources of MRS medium and after optimization of pH and temperature using date juice as a C-source. Plackett-Burman design showed peptone, K2HPO4, sodium acetate and date juice as significant components influencing the lactic acid production.
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