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[Angiogenesis VEGF165 gene therapy with AdVEGF- a new delay procedure for flaps]
T Holzbach1, C Taskov, I Neshkova
1Abteilung für Plastische und Wiederherstellungschirurgie, Klinikum rechts der Isar, Technische Universität München.
Handchirurgie, Mikrochirurgie, Plastische Chirurgie : Organ Der Deutschsprachigen Arbeitsgemeinschaft Fur Handchirurgie : Organ Der Deutschsprachigen Arbeitsgemeinschaft Fur Mikrochirurgie Der Peripheren Nerven Und Gefasse : Organ Der V
|January 3, 2006
Summary
Vascular Endothelial Growth Factor (VEGF) gene therapy significantly improved flap survival and perfusion in rats. This suggests VEGF therapy may be a superior alternative to the surgical "Delay" procedure for enhancing blood vessel formation.
Area of Science:
- Biomedical Engineering
- Molecular Biology
- Plastic Surgery
Context:
- Adequate blood supply is crucial for tissue function, with growth factors regulating blood vessel formation.
- The "Delay" procedure, a sublethal ischemia, is used in plastic surgery to stimulate angiogenesis, but its molecular mechanisms are unclear.
- Vascular Endothelial Growth Factor (VEGF) plays a key role in angiogenesis and its regulation by oxygen suggests a link to the "Delay" phenomenon.
Purpose:
- To investigate the role of VEGF in the "Delay" phenomenon and compare VEGF gene therapy to the "Delay" procedure in enhancing flap survival.
- To measure VEGF concentration and flap perfusion in response to VEGF gene therapy and the "Delay" procedure.
- To analyze the impact of these interventions on the survival and necrosis of random pattern skin flaps.
Summary:
- VEGF concentrations were measured in rat skin flaps following VEGF gene therapy or the "Delay" procedure.
- Both "Delay" and VEGF gene therapy increased local VEGF levels in skin, but only VEGF gene therapy significantly improved flap perfusion and survival.
- The underlying muscle showed no significant VEGF changes between groups.
Impact:
- VEGF gene therapy offers a promising approach to enhance flap perfusion and reduce necrosis in reconstructive surgery.
- Understanding the molecular mechanisms of VEGF in angiogenesis can lead to improved surgical outcomes.
- VEGF gene therapy demonstrated superior results compared to the "Delay" procedure in this experimental model.