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[Construction of tumor cell-specific expression vector modulated by human telomerase reserse transcription gene core
Hong-mei Li1, Tian-bao Song, Yue-cheng Yu
1Environment and Genes Related to Diseases Key Laboratory of Education Ministry, School of Medicine, Xi'an Jiaotong University, Xi'an 710061, China. ylhmqq@yahoo.com.cn
Aim:
To construct the tumor cell-specific expression vector modulated by human telomerase reserse transcription gene (hTERT) core promoter.
Methods:
The hTERT gene core promoter fragment was amplified by PCR using the total genomic DNA from SKOV3 cells as template. The amplified gene fragment was subsequently cloned into pIRES2-EGFP vector. Then the expression vector modulated by hTERT core gene promoter was transfected into tumor cell lines, HO-8910, Hela and normal ECV304 cells through lipofectamine, respectively. The activity of reporter gene GFP was detected 48 h after transfection.
Results:
There was significant transcriptional activity in 3 kinds of transfected tumor cells, while no transcriptional activity was detected in transfected normal cells.
Conclusion:
The hTERT core gene promoter has the tumor specificity, suggesting that the specific expression vector modulated by hTERT core gene promoter may be a novel and promising approach to the tumor treatment.
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