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Related Experiment Videos

Operator experience and sample quality in genetic amniocentesis.

Robert A Welch1, Soha Salem-Elgharib, Anne E Wiktor

  • 1Providence Hospital, Southfield, MI 48075, USA.

American Journal of Obstetrics and Gynecology
|January 4, 2006
PubMed
Summary

Physician experience impacts maternal cell contamination rates during genetic amniocentesis. Lower annual volumes (<50 procedures) correlate with a higher frequency of contamination in prenatal diagnostic samples.

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Area of Science:

  • Prenatal Diagnostics
  • Cytogenetics
  • Reproductive Medicine

Background:

  • Maternal cell contamination (MCC) can compromise the accuracy of cytogenetic analysis in amniotic fluid samples.
  • Physician experience is a potential factor influencing MCC rates, but data is limited.

Purpose of the Study:

  • To investigate the relationship between the annual volume of genetic amniocentesis procedures performed by a physician and the incidence of MCC.
  • To determine if a threshold of physician experience exists for minimizing MCC in prenatal samples.

Main Methods:

  • A retrospective review of amniotic fluid samples submitted for cytogenetic analysis between 2000 and 2004 was conducted.
  • Samples with male karyotypes (46 XY) were analyzed for MCC, identified by the presence of female cells (46 XX).

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  • Physicians were categorized based on annual sample submission volume: >= 50 samples versus < 50 samples.

    • Main Results:

    • A total of 6332 samples yielding male karyotypes were analyzed.
    • Physicians submitting < 50 samples annually had a significantly higher MCC rate (0.67%) compared to those submitting >= 50 samples (0.19%).
    • This difference was statistically significant (P = .0021).

    Conclusions:

    • Physician experience, quantified by annual amniocentesis volume, is inversely related to MCC rates.
    • Performing fewer than 50 genetic amniocenteses annually is associated with an increased risk of maternal cell contamination in prenatal samples.