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Thyroid albumin originates from blood.

J J de Vijlder1, G J Veenboer, J E van Dijk

  • 1Department of Experimental Pediatric Endocrinology, University of Amsterdam, The Netherlands.

Endocrinology
|August 1, 1992
PubMed
Summary

Thyroid glands in goats with goiter do not synthesize albumin. Instead, this "thyralbumin" originates from the blood, indicating a defect in thyroglobulin synthesis and serum protein passage into the thyroid.

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Area of Science:

  • Endocrinology
  • Molecular Biology
  • Veterinary Medicine

Background:

  • Goiter in Dutch goats is associated with a thyroglobulin synthesis defect.
  • Increased follicle presence of albumin was observed in goitrous thyroids compared to normal glands.

Purpose of the Study:

  • To investigate the origin of
  • thyralbumin
  • found in goitrous goat thyroids.
  • To determine if the thyroid gland synthesizes albumin or if it is derived from serum proteins.

Main Methods:

  • Immunohistochemical analysis of thyroid follicles for albumin, transferrin, and immunoglobulin G.
  • In vivo labeling with 125I and in vitro incubation with [3H] leucine.
  • Cyanogen bromide peptide mapping of albumin.
  • Detection of albumin mRNA using Northern blotting and polymerase chain reaction (PCR).

Main Results:

  • Albumin in goitrous glands did not incorporate [3H] leucine, suggesting no de novo synthesis.
  • Albumin from goiter and serum albumin showed similar cyanogen bromide cleavage patterns.
  • No albumin mRNA was detected in total RNA from normal or goitrous goat thyroids.

Conclusions:

  • The
  • thyralbumin
  • found in goat goiter originates from the blood serum.
  • The thyroid gland does not synthesize albumin; its presence is due to intercellular passage of serum proteins.

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