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Mobile nucleosomes--a general behavior.

G Meersseman1, S Pennings, E M Bradbury

  • 1Department of Biological Chemistry, School of Medicine, University of California, Davis 95616.

The EMBO Journal
|August 1, 1992
PubMed
Summary

Nucleosomes exhibit temperature-dependent sliding on DNA, a dynamic behavior potentially common across various sequences and histone octamer positions. This short-range sliding is influenced by DNA sequence and histone interactions.

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Area of Science:

  • Molecular Biology
  • Genetics
  • Biochemistry

Background:

  • Nucleosomes are fundamental units of DNA packaging in eukaryotes.
  • Nucleosome positioning and dynamics are crucial for gene regulation.
  • Previous work established nucleosome mobility on sea urchin 5S rDNA.

Purpose of the Study:

  • To investigate the temperature dependence and range of nucleosome mobility.
  • To determine if this mobility is a general phenomenon.
  • To explore the influence of DNA sequence and histone octamer position.

Main Methods:

  • Nucleoprotein gel electrophoresis was employed.
  • Analysis of nucleosome mobility on 5S rDNA constructs.
  • Reconstitution of nucleosomes onto Alu family repeats.

Main Results:

  • Nucleosome mobility on 5S rDNA is temperature-dependent.
  • Short-range sliding was observed in bulk mononucleosomes and reconstituted nucleosomes.
  • Histone octamer location significantly impacts electrophoretic migration.

Conclusions:

  • Short-range nucleosome sliding is a potentially general phenomenon.
  • Sequence-dependent and position-dependent factors influence nucleosome dynamics.
  • Nucleoprotein gel electrophoresis is a valuable tool for studying nucleosome positioning and dynamics.

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