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Multiplex PCR Assay for Typing of Staphylococcal Cassette Chromosome Mec Types I to V in Methicillin-resistant Staphylococcus aureus
Published on: September 5, 2013
Novel PCR-restriction fragment length polymorphism analysis for rapid typing of staphylococcal cassette chromosome
Jin Ah Yang1, Dae Won Park, Jang Wook Sohn
1Graduate School of Life Science and Biotechnology, College of Medicine, Korea University, Seoul.
Abstract:
We developed a novel PCR-restriction fragment length polymorphism test for the ccrB gene by using HinfI and BsmI for rapid typing of staphylococcal cassette chromosome mec (SCCmec). When tested with reference strains and methicillin-resistant Staphylococcus aureus isolates, the method proved to be valid and useful for rapid identification of four SCCmec types, especially type IV.
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