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Related Experiment Videos

Monocyte-derived CXCL7 peptides in the marrow microenvironment.

Manoj M Pillai1, Mineo Iwata, Norihiro Awaya

  • 1Fred Hutchinson Cancer Research Center, 1100 Fairview Ave N, D1-100, PO Box 19024, Seattle, WA 98109-1024, USA.

Blood
|January 5, 2006
PubMed
Summary

Stromal cells induce monocytes to express CXCL7, a protein previously thought to be specific to megakaryocytes. This finding suggests a new role for monocytes in regulating bone marrow megakaryocytopoiesis.

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Area of Science:

  • Hematology
  • Cell Biology
  • Molecular Biology

Background:

  • The bone marrow microenvironment comprises diverse interacting cells, including monocytes/macrophages and stromal cells.
  • Gene expression differs when these cell types are cultured together versus separately.

Purpose of the Study:

  • To investigate the expression of CXCL7 in monocytes cocultured with stromal cells.
  • To determine the functional role of CXCL7 and related proteins in megakaryocytopoiesis.

Main Methods:

  • Quantitative reverse transcriptase-polymerase chain reaction (RT-PCR) for gene expression analysis.
  • Enzyme-linked immunosorbent assay (ELISA) and Western blot for protein detection.
  • Coculture assays with stromal cells and monocytes, immunohistochemistry on bone marrow biopsies.

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Main Results:

  • CXCL7 gene expression was observed in monocytes cocultured with stromal cells, contrary to previous understanding.
  • Stromal cells provided at least two activities necessary for optimal CXCL7 expression by monocytes.
  • The CXCL7 form NAP-2 reduced the size and number of CFU-Meg colonies.
  • Monocytes were identified as an in vivo source of CXCL7 in bone marrow.

Conclusions:

  • Stromal-stimulated monocytes are a significant source of CXCL7 peptides within the bone marrow microenvironment.
  • Monocytes may play a role in the local regulation of megakaryocytopoiesis through CXCL7 production.