Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A rat beta-interferon-induced mRNA: sequence characterization.

D J Hayzer1, E Brinson, M S Runge

  • 1Division of Cardiology, Emory University School of Medicine, Atlanta, GA 30322.

Gene
|August 15, 1992
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

In situ hybridization.

Methods in molecular medicine·2011
Same author

Western blotting.

Methods in molecular medicine·2011
Same author

Strategies for the design of novel thrombolytic and antithrombolytic agents.

Trends in cardiovascular medicine·2011
Same author

Oxidative stress in atherogenesis and arterial thrombosis: the disconnect between cellular studies and clinical outcomes.

Journal of thrombosis and haemostasis : JTH·2005
Same author

The no-reflow phenomenon in coronary arteries.

Journal of thrombosis and haemostasis : JTH·2004
Same author

Construction and characterization of a recombinant plasminogen activator composed of an anti-fibrin single-chain antibody and low-molecular-weight urokinase.

Journal of thrombosis and haemostasis : JTH·2004
Same journal

Face/off: phase-specific modeling of lineage plasticity using near-patient models in genitourinary cancers.

Gene·2026
Same journal

Hierarchical analysis of metabolic phenotype reveals distinct microbiota and circulatory transcriptome in metabolic dysfunction-associated steatotic liver disease.

Gene·2026
Same journal

Mutation T71R enhanced the structural stability and functional activity of wild type superoxide dismutase cloned from soil metagenome.

Gene·2026
Same journal

Reduced ATXN1 expression as an adverse prognostic indicator in Acute myeloid leukemia.

Gene·2026
Same journal

Constructing regulatory networks of Rubisco post-translational modifications: a novel avenue for engineering environment adaptive plants.

Gene·2026
Same journal

Traumatic brain injury enhances fracture healing by upregulating VNN1 to activate the Wnt/β-catenin signaling pathway.

Gene·2026
See all related articles

Researchers identified a novel rat gene, rat8, through PCR amplification. This gene shows significant similarity to human interferon-inducible genes, suggesting a conserved role in cellular response.

Area of Science:

  • Molecular Biology
  • Genetics
  • Immunology

Background:

  • Adrenergic receptors play crucial roles in smooth muscle function.
  • Interferon-inducible genes are involved in cellular defense mechanisms.

Purpose of the Study:

  • To identify and characterize novel genes in rat aortic smooth muscle cells.
  • To investigate potential similarities between rat and human gene families.

Main Methods:

  • Polymerase chain reaction (PCR) amplification of cDNA from rat aortic smooth muscle cells.
  • Sequence analysis and comparison with known human genes.
  • Southern blot analysis of rat DNA.

Main Results:

  • A novel clone, designated rat8, was isolated.

Related Experiment Videos

  • Rat8 exhibits high sequence similarity (70%) to human alpha-interferon (IFN)-induced gene 9-27.
  • Deduced amino acid sequences show 66% similarity between rat8 and its human counterpart.
  • Southern analysis indicates rat8 is part of a large multigene family in rats.

    • Conclusions:

    • Rat8 represents a potential homologue to human interferon-inducible genes.
    • The findings suggest a conserved evolutionary pathway for interferon-inducible genes in mammals.
    • Rat8's multigene nature implies a complex regulatory role in rat physiology.