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Updated: Jul 30, 2026

Serum and Plasma Copy Number Detection Using Real-time PCR
Published on: December 15, 2017
Real-time PCR analysis of trinucleotide repeat allele expansions in the androgen receptor gene
Anthoula Chatzikyriakidou1, Christos Yapijakis, Nikolaos Sofikitis
1Genetics Unit, Department of Obstetrics and Gynaecology, Ioannina University School of Medicine, Ioannina, Greece.
Introduction:
The expansion of specific trinucleotide repeats results in certain genetic disorders.
Method:
Real-time PCR analysis was used to rapidly discriminate between normal and expanded (CAG)(n) alleles in the androgen receptor gene.
Result:
The difference in melting temperature (T(m)) between the most common normal and expanded alleles was approximately 1 degrees C.
Conclusion:
Real-time PCR analysis seems to be a highly reliable and rapid method, which may facilitate the first molecular approach to human trinucleotide repeat disorders.
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