Real-time PCR analysis of trinucleotide repeat allele expansions in the androgen receptor gene
Related Experiment Video
Updated: Jul 30, 2026

Serum and Plasma Copy Number Detection Using Real-time PCR
Published on: December 15, 2017
Anthoula Chatzikyriakidou1, Christos Yapijakis, Nikolaos Sofikitis
1Genetics Unit, Department of Obstetrics and Gynaecology, Ioannina University School of Medicine, Ioannina, Greece.
Introduction:
The expansion of specific trinucleotide repeats results in certain genetic disorders.
Method:
Real-time PCR analysis was used to rapidly discriminate between normal and expanded (CAG)(n) alleles in the androgen receptor gene.
Result:
The difference in melting temperature (T(m)) between the most common normal and expanded alleles was approximately 1 degrees C.
Conclusion:
Real-time PCR analysis seems to be a highly reliable and rapid method, which may facilitate the first molecular approach to human trinucleotide repeat disorders.
More Related Videos
Related Concept Videos
PCR - Polymerase Chain Reaction
Real Time RT-PCR
The real-time quantification of the number of amplified products is...
RACE - Rapid Amplification of cDNA Ends
Since the...

