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Related Experiment Videos

Single- and multi-photon excited fluorescence from serotonin complexed with beta-cyclodextrin.

Roger H Bisby1, Stanley W Botchway, Shakeela Dad

  • 1Biosciences Research Institute, University of Salford, Salford, UK. r.h.bisby@salford.ac.uk

Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology
|January 6, 2006
PubMed
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Serotonin

Area of Science:

  • Biochemistry
  • Analytical Chemistry
  • Spectroscopy

Background:

  • Serotonin, a key neurotransmitter, exhibits fluorescence properties.
  • Beta-cyclodextrin is a cyclic oligosaccharide used for molecular encapsulation.
  • Understanding their interaction is crucial for sensitive detection methods.

Purpose of the Study:

  • To investigate the fluorescence changes of serotonin upon binding with beta-cyclodextrin.
  • To characterize the unique 'hyperluminescence' phenomenon of serotonin under multiphoton excitation.
  • To assess the potential of these findings for serotonin detection and imaging.

Main Methods:

  • Steady-state and time-resolved fluorescence spectroscopy.
  • Multiphoton near-infrared excitation with sub-picosecond pulses.

Related Experiment Videos

  • Analysis of fluorescence intensity, lifetime components, and binding constants.

    • Main Results:

    • Serotonin fluorescence intensity increased by ~30% upon beta-cyclodextrin binding, with a binding constant (K(A)) of ~60 dm(3) mol(-1).
    • Fluorescence lifetimes of serotonin increased upon complexation.
    • Multiphoton excited 'hyperluminescence' resolved into two lifetime components, attributed to free and bound serotonin.

    Conclusions:

    • Beta-cyclodextrin binding significantly alters serotonin's fluorescence characteristics.
    • Hyperluminescence provides a sensitive method for distinguishing free and bound serotonin.
    • These findings support selective serotonin imaging and detection, particularly when using capillary electrophoresis with cyclodextrins.