[Study on BS-12 as a new probe for the determination of DNA by resonance light scattering]

A new method has been developed for the determination of DNA by resonance light scattering with dodecyl dimethyl betaine (BS-12) in aqueous solution as a new probe. At pH 9.3, the interactions of BS-12 and DNA gave strong RLS signals at 388.0 nm. Linear relationships were found between the enhanced intensity of RLS and the concentration of DNAs in the range 0.25-12.0 microg x mL(-1) for fsDNA and 0.25-11.0 microg x mL(-1) for ctDNA. The limits of detection were 0.15 ng x mL(-1) and 0.16 ng x mL(-1) for fsDNA and ctDNA, respectively. The method was applied to the determination of synthetic samples with satisfactory results.