Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Murine strain differences in contractile function are temperature- and frequency-dependent.

Linda B Stull1, Nitisha Hiranandani, Missy A Kelley

  • 1The Institute of Molecular Cardiobiology, Johns Hopkins University School of Medicine, 720 Rutland Avenue, Baltimore, MD 21205, USA.

Pflugers Archiv : European Journal of Physiology
|January 7, 2006
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Remembering James Forrester: A Legend of Cardiology.

Journal of the American College of Cardiology·2026
Same author

Moving Beyond the Hype in Extracellular Vesicle Therapy.

JACC. Basic to translational science·2026
Same author

Exosome-Mediated Benefits of Cell Therapy in Mouse and Human Models of Duchenne Muscular Dystrophy.

Stem cell reports·2026
Same author

Small noncoding RNA TY2 enhances efferocytosis and improves outcomes in a mouse model of sepsis.

JCI insight·2026
Same author

Cardiosphere-derived cells in the primary prevention of sepsis-induced acute lung injury in pigs.

PloS one·2026
Same author

Multicenter HFpEF study identifies sex disparity linked with two discrete cardiac proteomic signatures.

bioRxiv : the preprint server for biology·2025

Cardiac contractile function is similar across mouse strains under physiological conditions. However, non-physiological conditions reveal strain-dependent differences, impacting research findings.

Area of Science:

  • Cardiovascular Physiology
  • Comparative Myology
  • Animal Models in Research

Background:

  • Mice are crucial for cardiac research, yet strain-specific variations in cardiac function remain understudied.
  • Understanding these variations is vital for accurate interpretation of experimental results.

Purpose of the Study:

  • To investigate and compare basic cardiac contractile function in isolated right ventricular trabeculae from three common mouse strains.
  • To determine the influence of physiological versus sub-physiological conditions on strain-dependent cardiac performance.

Main Methods:

  • Isolated multicellular right ventricular trabeculae from C57BL/6, SV129, and FVBN mice were studied.
  • Contractile function was assessed under conditions mimicking in vivo (37°C, 8-12 Hz) and sub-physiological settings.

Related Experiment Videos

  • Responses to beta-adrenergic stimulation were evaluated.

    • Main Results:

    • Trabeculae isolation frequency varied significantly between strains (FVBN/SV129 > C57BL/6).
    • Under near physiological conditions, contractile function and beta-adrenergic response were similar across all strains.
    • At sub-physiological frequency and temperature, contractile performance differed notably between strains, with FVBN showing greater force development.

    Conclusions:

    • Basic cardiac contractile function is conserved across common mouse strains under physiological conditions.
    • Sub-physiological experimental conditions can introduce significant strain-dependent variability in cardiac performance.
    • Careful consideration of mouse strain is essential, particularly when extrapolating findings from non-physiological experimental settings to in vivo cardiac function.