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A temperature-sensitive calmodulin mutant loses viability during mitosis.

¹Department of Biochemistry, University of Washington, Seattle.

The Journal of Cell Biology

|August 1, 1992

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A rare temperature-sensitive calmodulin mutant in yeast (Saccharomyces cerevisiae) exhibits severe cell cycle defects, leading to lethality due to chromosome mis-segregation and failed cytokinesis at non-permissive temperatures.

Area of Science:

Molecular Biology
Cell Biology
Genetics

Background:

Calmodulin is a crucial calcium-binding protein involved in numerous cellular processes.
Mutations in calmodulin can lead to significant cellular dysfunction.

Purpose of the Study:

To characterize a novel recessive temperature-sensitive calmodulin mutant in Saccharomyces cerevisiae.
To investigate the cellular defects associated with this calmodulin mutation.

Main Methods:

Isolation and characterization of a temperature-sensitive calmodulin mutant (CMD1).
Analysis of cell cycle progression, viability, DNA segregation, and cytokinesis at permissive and non-permissive temperatures.
Assessment of chromosome loss rates.

Main Results:

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The mutant, with isoleucine 100 to asparagine and glutamic acid 104 to valine substitutions, displayed temperature-sensitive lethality.
Defects in nuclear movement, chromosome segregation, and cytokinesis were observed at non-permissive temperatures.
Increased chromosome loss rates were detected at semi-permissive temperatures, indicating defects in chromosome disjunction.

Conclusions:

The identified calmodulin mutations disrupt essential cell cycle processes, including nuclear migration and chromosome segregation.
This mutant provides a valuable tool for studying calmodulin function in cell division and chromosome stability in yeast.