Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Competition between cell-substratum interactions and cell-cell interactions.

P S Tung1, K Burdzy, K Wong

  • 1Banting and Best Department of Medical Research, University of Toronto, C.H. Best Institute, Ontario, Canada.

Journal of Cellular Physiology
|August 1, 1992
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Measurement of charged particle spectra in minimum-bias events from proton-proton collisions at <math> </math>.

The European physical journal. C, Particles and fields·2019
Same author

Measurement of the weak mixing angle using the forward-backward asymmetry of Drell-Yan events in <math></math> collisions at 8 <math></math>.

The European physical journal. C, Particles and fields·2019
Same author

Search for single production of vector-like quarks decaying to a top quark and a <math></math> boson in proton-proton collisions at <math> </math>.

The European physical journal. C, Particles and fields·2019
Same author

Search for the Higgs Boson Decaying to Two Muons in Proton-Proton Collisions at sqrt[s]=13  TeV.

Physical review letters·2019
Same author

Search for Leptoquarks Coupled to Third-Generation Quarks in Proton-Proton Collisions at sqrt[s]=13  TeV.

Physical review letters·2019
Same author

Evidence for the Associated Production of a Single Top Quark and a Photon in Proton-Proton Collisions at sqrt[s]=13  TeV.

Physical review letters·2018

Clusterin protein promotes cell aggregation, but its effect on TM4 cells depends on cell-substratum interactions. It aggregates TM4 cells when cell-adhesion is weak, and YIGSR peptide inhibits this response.

Area of Science:

  • Cell Biology
  • Biochemistry
  • Biophysics

Background:

  • Clusterin is a glycoprotein known to induce cell aggregation.
  • Understanding factors that modulate cell-cell vs. cell-substratum interactions is crucial in cell biology.
  • Anchorage-dependent and -independent cells exhibit distinct adhesion properties.

Purpose of the Study:

  • To investigate how clusterin influences the balance between cell-substratum and cell-cell interactions.
  • To compare the response of anchorage-independent erythrocytes and anchorage-dependent TM4 cells to clusterin under varying substratum conditions.
  • To elucidate the role of laminin receptors in mediating clusterin's effects on TM4 cell aggregation.

Main Methods:

  • Culturing erythrocytes and TM4 cells on laminin-coated (strong interaction) and agarose-coated (weak interaction) substrata.

Related Experiment Videos

  • Treating cells with clusterin and observing aggregation responses.
  • Utilizing the YIGSR peptide to block laminin receptors and assess its impact on TM4 cell behavior.
  • Analyzing the effect of clusterin and YIGSR on TM4 cell attachment to laminin.

    • Main Results:

    • Clusterin-induced erythrocyte aggregation was independent of the substratum type.
    • Clusterin aggregated TM4 cells only when cell-substratum interactions were weakened (on agarose).
    • YIGSR peptide inhibited clusterin-induced TM4 cell aggregation and prevented TM4 cell attachment to laminin, an effect reversed by clusterin.

    Conclusions:

    • Clusterin's ability to promote cell-cell interactions (aggregation) is context-dependent, particularly for anchorage-dependent cells.
    • Laminin receptors play a significant role in mediating the competition between cell-substratum adhesion and clusterin-induced cell-cell interactions in TM4 cells.
    • Clusterin may modulate cell adhesion dynamics by influencing the laminin receptor pathway.