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Treatment with Met-RANTES decreases bacterial translocation in experimental colitis
Can Kucuk1, Erdogan Sozuer, Sebnem Gursoy
1Department of General Surgery, Erciyes University School of Medicine, 38039, Kayseri, Turkey. cankucuk@erciyes.edu.tr
Background:
During colitis, epithelial function is impaired, leading to increased bacterial translocation. Recent studies have shown the important role of proinflammatory cytokines and chemokines, including RANTES (regulated on activation, normal T-cell expressed and secreted), in inflammatory bowel diseases (IBDs). In this study, we evaluated the role of Met-RANTES, an antagonist of the RANTES receptor, on the impairment of bacterial translocation in a rat model of colitis.
Methods:
Rats were randomly assigned to 3 groups. Group 1 = control, group 2 = experimental colitis, and group 3 = colitis plus Met-RANTES treatment. On day 7 after colitis was induced, plasma tumor necrosis factor-alpha colon tissue myeloperoxidase and portal blood endotoxin levels were measured. Lymph node, liver, and spleen culture quantified bacterial translocation.
Results:
Met-RANTES treatment resulted in significant decreases in colonic damage as well as bacterial translocation in experimental colitis.
Conclusions:
These results suggest that chemokine receptor antagonists may potentially be useful in the treatment of IBDs.
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