Related Experiment Video
Updated: Aug 13, 2026
11:54
Absorption of Nasal and Bronchial Fluids: Precision Sampling of the Human Respiratory Mucosa and Laboratory Processing of Samples
Published on: January 21, 2018
Malondialdehyde level and adenosine deaminase activity in nasal polyps
Erdogan Okur1, Fatma Inanc, Ilhami Yildirim
1Department of Otorhinolaryngology, School of Medicine, Kahramanmaras Sutcu Imam University, Hastane Cad. No. 32, 46050-Kahramanmaras, Turkey. erdoganokur@yahoo.com
Summary
Nasal polyps show significantly higher adenosine deaminase (ADA) activity and malondialdehyde (MDA) levels compared to normal nasal tissue. This suggests a role for ADA and oxidative stress in nasal polyp development.
Area of Science:
- Biochemistry
- Otorhinolaryngology
- Pathology
Background:
- Adenosine deaminase (ADA) is linked to neutrophil-derived free radical production.
- Malondialdehyde (MDA) is a marker of lipid peroxidation and tissue damage.
- Limited data exists on ADA activity in nasal mucosa and polyps.
Purpose of the Study:
- To determine and compare ADA activity and MDA levels in nasal polyps versus normal nasal mucosa.
- To investigate the relationship between ADA activity and MDA levels in nasal polyps.
Main Methods:
- Tissue samples from 23 nasal polyp patients and 14 controls (septal deviation/turbinate hypertrophy) were analyzed.
- MDA levels were quantified using the Okawa method (modified).
- ADA activity was measured using the Giusti method.
Main Results:
- Nasal polyp tissue exhibited significantly higher MDA levels (2.43 ± 0.38 nmol/mg protein) and ADA activity (0.235 ± 0.055 U/mg protein) compared to control mucosa (MDA: 1.03 ± 0.41, ADA: 0.056 ± 0.011; P < 0.05).
- A significant positive correlation was observed between MDA levels and ADA activity in nasal polyps (r = 0.701, P < 0.001).
Conclusions:
- Adenosine deaminase activity is detectable in nasal mucosa.
- Nasal polyp tissue shows significantly elevated MDA levels and ADA activity compared to normal turbinate tissue.
- These findings suggest increased oxidative stress and ADA involvement in nasal polyp pathogenesis.
