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Optimizing the heterologous expression of glutathione transferase
1Department of Biochemistry, Uppsala University Biomedical Center, Sweden.
Abstract:
The heterologous expression of a protein may be enhanced by silent mutations in the coding region of its corresponding DNA. This simple approach has been successfully used for optimized production of a number of glutathione-linked enzymes. For example, the yield of human glutathione transferase M2-2 was elevated by 140-fold in a clone isolated by immunoscreening of a library of plasmids with randomized synonymous codons in the 5'-segment of the region encoding the enzyme.
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