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Related Experiment Videos

Multivesicular release at Schaffer collateral-CA1 hippocampal synapses.

Jason M Christie1, Craig E Jahr

  • 1Vollum Institute, Oregon Health and Science University, Portland, Oregon 97239, USA. christij@ohsu.edu

The Journal of Neuroscience : the Official Journal of the Society for Neuroscience
|January 10, 2006
PubMed
Summary
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Multivesicular release (MVR) at synapses is linked to release probability. This study shows MVR occurs at Schaffer collateral-CA1 synapses when release probability increases, suggesting it

Area of Science:

  • Neuroscience
  • Synaptic transmission
  • Cellular biology

Background:

  • The mechanism of neurotransmitter release (single vs. multivesicular release) at individual synapses is debated.
  • Multivesicular release (MVR) may be regulated by the probability of neurotransmitter release (Pr).

Purpose of the Study:

  • To investigate neurotransmitter release at Schaffer collateral-CA1 synapses across varying release probabilities (Pr).
  • To determine if multivesicular release (MVR) is controlled by activity-dependent changes in Pr.

Main Methods:

  • Utilized patch-clamp recordings in acute hippocampal slices.
  • Estimated synaptic glutamate transient size using AMPA receptor EPSC inhibition with gamma-D-glutamylglycine.
  • Measured NMDA receptor EPSC duration under inhibited glutamate transporters.

Related Experiment Videos

Main Results:

  • Glutamate transient size correlated with Pr, not spillover, indicating simultaneous release from a single active zone.
  • Increased Pr prolonged NMDA receptor EPSCs when glutamate transporters were inhibited, consistent with an enhanced glutamate transient.
  • Multivesicular release (MVR) was observed at SC-CA1 synapses with elevated Pr due to facilitation.

Conclusions:

  • Multivesicular release (MVR) occurs at SC-CA1 synapses when release probability (Pr) is elevated.
  • MVR may be a widespread phenomenon across central nervous system (CNS) synapses.
  • Activity-dependent modulation of Pr can control MVR.