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Related Experiment Videos

Biodegradable polyurethane cytocompatibility to fibroblasts and staphylococci.

L G Harris1, K Gorna, S Gogolewski

  • 1AO Research Institute, AO Foundation, Clavadelerstrasse, 7270 Davos Platz, Switzerland. llinosharris@yahoo.co.uk

Journal of Biomedical Materials Research. Part A
|January 10, 2006
PubMed
Summary

Biodegradable polyurethanes (PUs) were tested for biocompatibility using fibroblast cells and bacteria. The 70% PU surface showed good fibroblast compatibility but supported bacterial adhesion, indicating potential risks for implantable devices.

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Area of Science:

  • Biomaterials Science
  • Polymer Chemistry
  • Cell Biology

Background:

  • Biodegradable polyurethanes (PUs) offer tunable properties for various implantable devices.
  • Assessing cytocompatibility and bacterial adhesion is crucial for evaluating in vivo suitability.

Purpose of the Study:

  • To determine the cytocompatibility of biodegradable polyurethanes with varying hydrophobic-to-hydrophilic ratios.
  • To investigate bacterial adhesion (Staphylococcus aureus, S. epidermidis) and human fibroblast (hTERT) cell behavior on these PUs.

Main Methods:

  • Synthesized biodegradable polyurethanes (PUs) with 100%, 70%, and 30% hydrophobic content.
  • Characterized PU surfaces using profilometry, contact angles, and SEM.
  • Assessed hTERT cell adhesion/spreading and bacterial adhesion on PU surfaces, PLDL, and Thermanox controls.

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Main Results:

  • Surface properties varied significantly among the tested PUs, PLDL, and Thermanox.
  • hTERT cells exhibited reduced spreading on 100% and 30% PU compared to 70% PU, PLDL, and Thermanox.
  • Bacterial adhesion was highest on 70% and 30% PU for S. aureus, and on 70% PU for S. epidermidis.

Conclusions:

  • The 70% PU surface demonstrated cytocompatibility with hTERT fibroblasts.
  • However, all tested PU surfaces, especially 70% PU, promoted bacterial colonization, posing a risk for implantable applications.