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Comparison of methodologies to measure human lung histamine
1Department of Internal Medicine, University of Iowa College of Medicine, Iowa City.
Journal of Immunological Methods
|July 31, 1992
Summary
Radioimmunoassay (RIA) is a superior method for measuring histamine in human lung samples. It accurately quantifies histamine across a wide range, from bronchoalveolar lavage fluid to lung tissue, aiding research on histamine and mast cell roles.
Area of Science:
- Pulmonary Medicine
- Biochemistry
- Analytical Chemistry
Background:
- Histamine and mast cells play crucial roles in lung physiology and pathology.
- Accurate measurement of histamine in human lung samples is essential for understanding these roles.
Purpose of the Study:
- To compare the effectiveness of different assays for histamine measurement in human lung samples.
- To identify the most suitable assay for quantifying histamine across various lung sample types and concentrations.
Main Methods:
- Comparison of a single isotope enzymatic assay, radioimmunoassay (RIA), and an automated spectrofluorometric method.
- Assays were evaluated for their ability to measure histamine in bronchoalveolar lavage fluid and human lung tissue.
Main Results:
- Both enzymatic assay and RIA accurately measured low histamine concentrations (0.05-2.0 ng/ml) in bronchoalveolar lavage fluid.
- RIA effectively measured high histamine levels (up to 1500 ng/ml) in lung tissue, outperforming the spectrofluorometric method in this range.
- RIA demonstrated a broader effective measurement range (0.05-1500 ng/ml) compared to the other assays.
Conclusions:
- Radioimmunoassay (RIA) is a versatile and accurate method for histamine quantification in diverse human lung samples.
- RIA's ease of performance, cost-effectiveness, and wide dynamic range make it preferable for research on histamine and mast cell functions in the lung.
- This assay facilitates further elucidation of histamine's role in normal and diseased lung states.