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Bone marrow-derived cells in normal human corneal stroma.

Satoru Yamagami1, Nobuyuki Ebihara, Tomohiko Usui

  • 1Departments of Corneal Tissue Regeneration and Ophthalmology, University of Tokyo Graduate School of Medicine, Hongo 7-3-1, Bunkyo-ku, Tokyo, Japan 113-8655. syamagami-tky@umin.ac.jp

Archives of Ophthalmology (Chicago, Ill. : 1960)
|January 13, 2006
PubMed
Summary
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Bone marrow-derived monocyte lineage cells, identified by CD45 and HLA-DR expression, are present in the normal human cornea. These cells may contribute to corneal immune responses.

Area of Science:

  • Ophthalmology
  • Immunology
  • Cell Biology

Background:

  • The human cornea's immune privilege is well-established.
  • The presence and role of bone marrow-derived cells within the corneal stroma remain incompletely understood.

Purpose of the Study:

  • To investigate the presence and characteristics of bone marrow-derived cells in the normal human corneal stroma.
  • To determine if these cells express toll-like receptor 4 (TLR4).

Main Methods:

  • Immunohistochemistry and fluorescent microscopy were used to examine 34 human corneas.
  • CD45-positive cells were isolated using magnetic beads and analyzed for surface marker expression.
  • Toll-like receptor 4 expression was assessed via flow cytometry and RT-PCR.

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Main Results:

  • CD45-positive cells, indicative of monocyte lineage, were found in the anterior and peripheral corneal stroma.
  • These cells expressed CD11b, CD11c, CD14, and HLA-DR, but not markers for T cells, B cells, or NK cells.
  • Stromal CD45-positive cells, unlike CD45-negative cells, expressed toll-like receptor 4.

Conclusions:

  • Normal human corneas contain bone marrow-derived monocyte lineage cells expressing HLA-DR.
  • These cells are located in the anterior and peripheral stromal layers.
  • The presence of these cells and their expression of TLR4 suggest a role in corneal immune surveillance and response.