Development of an expression vector for Metarhizium anisopliae based on the tef-1alpha homologous promoter
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Updated: Aug 13, 2026

Development of Metarhizium anisopliae as a Mycoinsecticide: From Isolation to Field Performance
Published on: July 30, 2017
Luciano Nakazato1, Valéria Dutra, Leonardo Broetto
1Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul, P.O. Box 15005, CEP 91501-970, Porto Alegre, RS, Brazil.
Abstract:
The high-conserved translation elongation factor 1 alpha (tef-1alpha) gene from the enthomopathogenic fungus Metarhizium anisopliae was characterized to select the promoter region. A 640-bp DNA fragment upstream to the start codon was employed to drive the expression of the reporter protein sGFP or a dominant selectable marker, the gene bar (resistance to ammonium glufosinate). Transformants carrying this homologous promoter system showed no difference in virulence bioassays against the cattle tick Boophilus microplus comparing to the M. anisopliae wild-type strain. Moreover, GFP fluorescence was detected during tick infection bioassay.

