Protein expression enhancement in efflux-deleted mutant bacteria

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Updated: Aug 13, 2026

Characterizing Multidrug Efflux Systems in Acinetobacter baumannii Using an Efflux-Deficient Bacterial Strain and a Single-Copy Gene Expression System
05:06

Characterizing Multidrug Efflux Systems in Acinetobacter baumannii Using an Efflux-Deficient Bacterial Strain and a Single-Copy Gene Expression System

Published on: January 5, 2024

Thuc T Le1, Călin C Guet, Philippe Cluzel

  • 1The Institute for Biophysical Dynamics, James Franck Institute, Center of Integrative Sciences Building, The University of Chicago, 929 E. 57th St, Chicago, IL 60637, USA.

Protein Expression and Purification
|January 13, 2006
PubMed

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The DNA replication, transcription, and translation processes are intricately coupled in bacteria, allowing efficient gene expression and rapid protein synthesis. While this physical and functional coordination is advantageous, it introduces challenges that bacteria overcome through specific regulatory mechanisms.Coupling of Replication, Transcription, and TranslationThe coupling of replication, transcription, and translation is a hallmark of bacterial gene expression. As the replisome unwinds...