Related Experiment Video
Updated: Aug 5, 2026
Determining Optimal Cytotoxic Activity of Human Her2neu Specific CD8 T cells by Comparing the Cr51 Release Assay to the xCELLigence System
Published on: August 8, 2012
The sensitivity of chemioresistant human tumor explants to lysis by activated and nonactivated autological
N M Berezhnaya1, U D Vinnichuk, V F Konovalenko
1R.E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology, NAS of Ukraine, Kyiv 03022, Ukraine. berezh@onconet.kiev.ua
Aim:
The comparative study of antitumor action of peripheral blood lymphocytes (PBL) and lymphokin-activated killer cells (LAK) on autologous cell of chemoresistant and chemosensitive human soft tissue sarcomas and tumors of epithelial origin.
Materials And Methods:
Tumor explants (15 samples of soft tissue sarcomas, 10 samples of cervical and ovarian carcinomas) were cultivated with autologous lymphocytes in double diffusion chambers. Evaluation of the results was done on the basis of morphological criteria of explants growth.
Results:
The results have shown that in the patients with the resistant soft tissue sarcomas and carcinomas LAK possess more pronounced antitumor action, than non-activated lymphocytes, whilst PBL possess higher antitumor action on sensitive epithelial tumors than that on soft tissue sarcoma.
Conclusion:
LAK possess antitumor action toward chemoresistant tumors of different origin and localization.
