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Published on: October 11, 2018
Cecal spirochetosis caused by Brachyspira pilosicoli in commercial turkeys
1California Animal Health and Food Safety Laboratory System, Fresno Branch University of California, Davis, 2789 S. Orange Avenue, Fresno, CA 93725, USA.
Abstract:
Spirochetes that were identified as Brachyspira pilosicoli were present in the ceca of 7.5- to 18-wk-old turkeys with cecal spirochetosis and typhlitis. The identity of B. pilosicoli was confirmed on the basis of ultrastructural morphology of the cecal epithelium adherent microbes, immunohistochemical staining with a Brachyspira genus-specific monoclonal antibody, and amplification of a B. pilosicoli species-specific 16S ribosomal RNA (rrs gene) sequence by using the polymerase chain reaction and DNA obtained by laser-capture microdissection of the epithelium-adherent microbial fringe. To the author's knowledge, this is the first report of B. pilosicoli in the ceca of turkeys.
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