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Electroporation as a tool to transfer the plasmid pRL489 in Oscillatoria MKU 277
C R Marutha Ravindran1, S Suguna, S Shanmugasundaram
1Department of Pharmacology, M.C. 7764, University of Texas Health Science Center, 7703 Floyd Curl Drive, San Antonio, TX 78229-3900, USA. ravindran@uthscsa.edu
Journal of Microbiological Methods
|January 13, 2006
Abstract:
To establish the gene transfer system in the cyanobacteria, we successfully introduced the shuttle plasmid pRL489 into Oscillatoria MKU 277 by electroporation with transformation frequencies of up to 102 cfu/mug of plasmid DNA. These findings suggest that Oscillatoria MKU 277 can be used as an experimental tool for genetic maneuvering.
