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Updated: Aug 13, 2026

High-throughput and Comprehensive Drug Surveillance Using Multisegment Injection-Capillary Electrophoresis-Mass Spectrometry
Published on: April 23, 2019
Implementation of a rapid and automated high performance liquid chromatography method development strategy for
Elizabeth F Hewitt1, Patrick Lukulay, Sergey Galushko
1Noramco, Inc., Chemical Services, 1440 Olympic Drive, Athens, GA 30601, USA. ehewitt@norus.jnj.com
Abstract:
A comprehensive, fully automated strategy is demonstrated for HPLC-UV chromatographic method development using ChromSword optimization software. The strategy involves: (1) the automated screening of various column and mobile phase combinations, (2) rational selection of the best starting conditions; and (3) subsequent automated method development to generate optimized separation methods. Pharmaceutical compounds were applied to solve problematic drug impurity separations. ChromSword software automates the screening, optimization, and documentation steps thus reducing the method development time. The strategy was compared to a manual method development approach showing the automated method strategy affords better selectivity in a shorter time.
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