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Updated: Aug 13, 2026
From Constructs to Crystals – Towards Structure Determination of β-barrel Outer Membrane Proteins
Published on: July 4, 2016
Mapping electrostatic potential of a protein on its hydrophobic surface: implications for crystallization of
Victor Sivozhelezov1, Eugenia Pechkova, Claudio Nicolini
1Fondazione Elba, P.zza SS. Apostoli 66, 00100 Rome, Italy.
Abstract:
Calculation and combined visualization of electrostatic and hydrophobic properties of Cytochrome P450scc based on two very different homology models allowed to identify extensive hydrophobic patches with neutral electrostatic potential and mutations removing such patches and thus expecting to facilitate crystallization of Cytochrome P450scc, especially for the nanotemplate crystallization method. Implications are discussed for optimizing crystallization and other aspects of protein surface properties and protein recognition.
