Related Experiment Video
Updated: Aug 13, 2026

Mass Spectrometry and Luminogenic-based Approaches to Characterize Phase I Metabolic Competency of In Vitro Cell Cultures
Published on: March 28, 2017
Interactive effects between CYP1A1 genotypes and environmental polychlorinated dibenzo-p-dioxins and dibenzofurans
Hsiu-Ling Chen1, Huey-Jen Su, Ying-Jan Wang
1Research Center of Environmental Trace Toxic Substances, Medical College, National Cheng Kung University, Tainan, Taiwan.
Abstract:
Previous studies reported that polychlorinated dibenzo-p-dioxins and dibenzofurans (PCDD/Fs) induced hepatic cytochrome P-4501A1 (CYP1A1). The aim of this study was to examine the interactive influence of CYP1A1 genotypes and PCDD/Fs exposure on liver function profile. PCDD/Fs levels and liver function parameters were determined in serum and correlated with genetic polymorphism of CYP1A1/Msp 1 in 225 human volunteers who had no or minimal occupational exposure to PCDD/F. The results showed that the highest glutamate pyruvate transaminase (GPT) activity levels were found in subjects with homozygous variant CYP1A1/Msp 1, followed by heterozygous variant, and finally homozygous wild type for those individuals whose serum PCDD/Fs levels were higher than 17.4 pg WHO-TEQ/g lipid. Data suggest that GPT activity levels may be modified by interaction of CYP1A1/Msp 1 genotype with dioxin after adjustment for age, alcohol consumption, and history of liver illness. Further studies are needed to characterize the variation in other related genes to verify whether a correlation exists between serum PCDD/Fs levels and adverse health effects.
Related Concept Videos
Pharmacogenetics of Phase I Enzymes: Cytochrome P450 Isozymes
Pharmacogenetic Phenotypes: Alterations in Pharmacokinetics, Drug Targets and Biologic Milieu
Pharmacogenetics of Drug Metabolism: Overview
Effect of Hepatic Disease on Pharmacokinetics: Drug Dosing and Hepatic Blood Flow
Effect of Hepatic Disease on Pharmacokinetics: Pathophysiologic Assessment and Liver Function Test
Pharmacogenetics of Phase II Enzymes: N-acetyltransferase, Thiopurine S-methyltransferase, UDP-glucuronosyltransferase
