Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Temporally staggered glomerulus development in the moth Manduca sexta.

Brian W Lipscomb1, Leslie P Tolbert

  • 1Arizona Research Laboratories Division of Neurobiology, University of Arizona, Tuscon, AZ 85721-0077, USA.

Chemical Senses
|January 13, 2006
PubMed
Summary

Olfactory receptor neuron (ORN) axons gradually form glomeruli in the antennal lobe (AL) over an extended developmental period. Later-arriving axons integrate into developing glomeruli, contributing to the final structure of olfactory processing units.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Astrocytic glutamate transport regulates a Drosophila CNS synapse that lacks astrocyte ensheathment.

The Journal of comparative neurology·2016
Same author

Activation of glial FGFRs is essential in glial migration, proliferation, and survival and in glia-neuron signaling during olfactory system development.

PloS one·2012
Same author

Development of a glial network in the olfactory nerve: role of calcium and neuronal activity.

Neuron glia biology·2011
Same author

Roles of glial cells in neural circuit formation: insights from research in insects.

Glia·2011
Same author

Localization of a GABA transporter to glial cells in the developing and adult olfactory pathway of the moth Manduca sexta.

The Journal of comparative neurology·2010
Same author

Roles of specific membrane lipid domains in EGF receptor activation and cell adhesion molecule stabilization in a developing olfactory system.

PloS one·2009

Area of Science:

  • Neuroscience
  • Developmental Biology
  • Olfactory System Research

Background:

  • Glomeruli are key structures in olfactory systems, formed by olfactory receptor neuron (ORN) axon terminals and central neuron dendrites.
  • ORN axons typically segregate into glomeruli based on odor specificity, serving as the primary units for odorant information processing.
  • Glomeruli develop from protoglomeruli, which are initial clusters of ORN axons synapsing onto dendrites.

Purpose of the Study:

  • To investigate the developmental timeline and integration process of ORN axons into glomeruli within the antennal lobe (AL).
  • To understand how later-arriving ORN axons, from distal antennal segments, incorporate into established or developing glomeruli.
  • To elucidate the temporal dynamics of glomeruli formation during metamorphic adult development.

Related Experiment Videos

Main Methods:

  • Observation of protoglomeruli development across metamorphic stages in the moth Manduca sexta.
  • Analysis of dendritic projections of serotonin-containing neurons within glomeruli at later developmental stages.
  • Labeling of ORN axons originating from proximal antennal segments to track their targeting within the AL.

Main Results:

  • Protoglomeruli present at stage 5 constitute only a small fraction of adult glomeruli, with numbers increasing in later stages.
  • Immature dendritic arbors were observed in developing glomeruli alongside more mature structures.
  • Early-arriving ORN axons from proximal segments appear to target a limited set of glomeruli.

Conclusions:

  • Antennal lobe (AL) glomeruli formation is an extended process, not completed by stage 5.
  • Later-arriving ORN axons integrate into glomeruli over time, potentially influenced by the expression of new odorant receptors.
  • The developmental timing of ORN axon arrival and targeting shapes the final glomerular architecture.