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In vitro and in vivo Bioluminescence Reporter Gene Imaging of Human Embryonic Stem Cells
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Imaging reporter genes for cell tracking with PET and SPECT

P D Acton1, R Zhou

  • 1Department of Radiology, Thomas Jefferson University, 796-G Main Building, 132 South 10th Street, Philadelphia, PA 19107, USA. paul.acton@jefferson.edu

The Quarterly Journal of Nuclear Medicine and Molecular Imaging : Official Publication of the Italian Association of Nuclear Medicine (AIMN) [And] the International Association of Radiopharmacology (IAR), [And] Section of the Society Of
|January 13, 2006
PubMed

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Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...