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Updated: Aug 13, 2026
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Renal Ischaemia Reperfusion Injury: A Mouse Model of Injury and Regeneration
Published on: June 7, 2014
Treatment with CO-RMs during cold storage improves renal function at reperfusion
A Sandouka1, B J Fuller, B E Mann
1Vascular Biology Unit, Department of Surgical Research, Northwick Park Institute for Medical Research, Harrow, Middlesex, UK.
Kidney International
|January 13, 2006
Summary
Carbon monoxide-releasing molecules (CO-RMs) protect kidneys from cold storage and ischemia-reperfusion injury by delivering carbon monoxide (CO). This improves renal function and mitochondrial respiration post-preservation.
Area of Science:
- Nephrology
- Transplantation Biology
- Pharmacology
Background:
- Low concentrations of carbon monoxide (CO) demonstrate protective effects against ischemia-reperfusion (I-R) injury.
- CO-releasing molecules (CO-RMs) are a novel class of compounds designed to deliver CO to biological systems, exerting pharmacological activities.
Purpose of the Study:
- To investigate the protective effects of CO released from CO-RMs against cold storage and I-R damage in isolated perfused kidneys.
- To assess the impact of CO-RMs on hemodynamic, biochemical, and mitochondrial parameters during kidney preservation and reperfusion.
Main Methods:
- Isolated perfused rabbit kidneys were flushed with CO-RMs (CORM-A1 or CORM-3) and stored at 4°C for 24 hours.
- Hemodynamic parameters (perfusion flow rate), glomerular filtration rate, sodium and glucose reabsorption, and mitochondrial respiration were measured post-storage and reperfusion.
- Guanylate cyclase inhibition (ODQ) and use of an inactive CO-RM (iCO-RM) were employed to confirm the mechanism of action.
Main Results:
- Kidneys treated with CO-RMs showed significantly improved perfusion flow rate, glomerular filtration rate, and sodium/glucose reabsorption compared to controls.
- CO-RM treatment increased the respiratory control index of kidney mitochondria.
- The protective effects were abolished when using an inactive CO-RM, confirming the role of CO delivery.
Conclusions:
- CO-releasing molecules are effective in delivering CO for kidney cold preservation.
- CO-RMs ameliorate vascular activity, energy metabolism, and renal function following reperfusion injury.
- CO-RMs represent a promising therapeutic strategy for improving organ preservation during transplantation.
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