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Updated: Aug 13, 2026

Ferric Chloride-induced Murine Thrombosis Models
Published on: September 5, 2016
[Effect of phenolic alkaloids of Menispermum dauricum on thrombosis and platelet aggregation]
1Department of Pharmacology, Tongji Medical College of Huazhong University of Science and Technology, Wuhan 430030, China. kong0051@hotmail.com
Aim:
To observe the effect of phenolic alkaloids of Menispermum dauricum (PAMD) on thrombosis and platelet aggregation, and to explore its mechanism of action.
Methods:
Thrombosis was observed with arteriovenous shunt thrombus model in rat; platelet aggregation was determined by Born's method; ultrastructure of platelet was observed by transmission electron microscope; TXB2 or 6-keto-PGF1alpha levels were assessed by radioimmunoassay; and NO was determined by colorimetric method.
Results:
PAMD dose-dependently inhibited experimental thrombus formation, platelet aggregation induced by ADP, AA and THR in vivo and ultrastructure changes stimulated by THR; PAMD increased the generation of 6-keto-PGF1alpha in thoracic aortae and NO level in plasma; and had no influence on TXB2 release (P > 0.05).
Conclusion:
PAMD inhibited thrombosis and platelet aggregation, and its mechanism might be due to the increase of PGI2 and NO level.
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