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Related Experiment Videos

Ergovaline movement across Caco-2 cells.

Nancy W Shappell1, David J Smith

  • 1USDA ARS Biosciences Research Laboratory, 1605 Albrecht Boulevard, Fargo, North Dakota 58105, USA. shappeln@fargo.ars.usda.gov

In Vitro Cellular & Developmental Biology. Animal
|January 18, 2006
PubMed
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Ergovaline and ergovalinine readily cross human intestinal cells intact, suggesting their potential role in fescue toxicosis. This study investigated ergot alkaloid absorption in Caco-2 cells.

Area of Science:

  • Pharmacology
  • Gastroenterology
  • Toxicology

Background:

  • Fescue toxicosis is a significant concern in livestock and potentially humans.
  • Ergovaline is a primary mycotoxin implicated in fescue toxicosis.
  • Understanding ergovaline's intestinal absorption is crucial for elucidating its toxicological mechanisms.

Purpose of the Study:

  • To investigate the intestinal absorption of ergovaline and its isomer, ergovalinine.
  • To determine the rate and extent of ergovaline/ergovalinine transport across human intestinal cells.
  • To model the movement of these ergot alkaloids within the intestinal environment.

Main Methods:

  • Utilized Caco-2 cells, a human colon carcinoma cell line, to model intestinal absorption.
  • Assessed ergovaline/ergovalinine movement across cell monolayers using high-performance liquid chromatography (HPLC).

Related Experiment Videos

  • Developed mathematical models to describe ergot alkaloid transport kinetics.

    • Main Results:

    • Ergovaline and ergovalinine isomers crossed intestinal cells intact and at comparable rates.
    • Cellular presence significantly reduced the rate of ergot alkaloid movement compared to basal conditions.
    • Accumulation in the basal compartment reached 25-40% after 6 hours, depending on initial concentrations.

    Conclusions:

    • Ergovaline and ergovalinine are readily absorbed by human intestinal cells.
    • Both isomers, individually or combined, may contribute to fescue toxicosis pathogenesis.
    • These findings highlight the potential systemic exposure to ergot alkaloids from dietary sources.