Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Rapid Identification of Pathogens

Rapid Identification of Pathogens

MALDI-TOF MS has transformed clinical microbiology by offering a rapid and reliable method for pathogen identification. The traditional approach to microbial identification typically involves time-consuming culture techniques and biochemical tests, which can delay the initiation of appropriate antimicrobial therapy. MALDI-TOF MS avoids these delays by using characteristic ribosomal protein mass patterns of microbial cells, enabling accurate species-level identification within minutes.Principle...

Automated Microbial Diagnostics

Automated Microbial Diagnostics

Automated diagnostic analyzers have transformed clinical microbiology by providing rapid and reliable methods for pathogen identification and antibiotic susceptibility testing. Among these systems, the Vitek 2 is widely used because it automates the traditionally labor-intensive processes of microbial identification (ID) and antibiotic susceptibility testing (AST), delivering standardized and timely results that are essential for effective patient care.Microbial Identification with ID CardsThe...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Functional divergence of white genes in Henosepilachna vigintioctopunctata revealed by RNA interference.

Insect molecular biology·2020

Same author

Silencing chitin deacetylase 2 impairs larval-pupal and pupal-adult molts in Leptinotarsa decemlineata.

Insect molecular biology·2018

Same author

The structural basis for the functional comparability of factor VIII and the long-acting variant recombinant factor VIII Fc fusion protein.

Journal of thrombosis and haemostasis : JTH·2017

Same author

Mechanism of feedback regulation of neutrophil inflammation in Henoch-Schönlein purpura.

European review for medical and pharmacological sciences·2016

Same author

Microangiopathic hemolytic anemia due to ADAMTS-13 loss in idiopathic systemic capillary leak syndrome.

Journal of thrombosis and haemostasis : JTH·2016

Same author

Arrangement and number of clustered regularly interspaced short palindromic repeat spacers are associated with erythromycin susceptibility in emm12, emm75 and emm92 of group A streptococcus.

Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases·2013

Search research articles

Related Experiment Video

Updated: Jul 12, 2026

Rapid Point-of-Care Assay of Enoxaparin Anticoagulant Efficacy in Whole Blood

Rapid Point-of-Care Assay of Enoxaparin Anticoagulant Efficacy in Whole Blood

Published on: October 12, 2012

A rapid enzyme-linked assay for ADAMTS-13.

J-J Wu¹, K Fujikawa, E C Lian

¹Department of Biochemistry, University of Washington, Seattle, WA 98195, USA.

Journal of Thrombosis and Haemostasis : JTH

|January 18, 2006

Summary

A new assay rapidly measures ADAMTS-13 activity, crucial for diagnosing thrombotic thrombocytopenic purpura. This method quantifies ADAMTS-13 levels and inhibitory antibodies in plasma efficiently.

More Related Videos

Click-Chemistry Based Fluorometric Assay for Apolipoprotein N-acyltransferase from Enzyme Characterization to High-Throughput Screening

Click-Chemistry Based Fluorometric Assay for Apolipoprotein N-acyltransferase from Enzyme Characterization to High-Throughput Screening

Published on: May 13, 2020

An Acetyl-Click Chemistry Assay to Measure Histone Acetyltransferase 1 Acetylation

An Acetyl-Click Chemistry Assay to Measure Histone Acetyltransferase 1 Acetylation

Published on: January 26, 2024

Related Experiment Videos

Last Updated: Jul 12, 2026

Rapid Point-of-Care Assay of Enoxaparin Anticoagulant Efficacy in Whole Blood

Rapid Point-of-Care Assay of Enoxaparin Anticoagulant Efficacy in Whole Blood

Published on: October 12, 2012

Click-Chemistry Based Fluorometric Assay for Apolipoprotein N-acyltransferase from Enzyme Characterization to High-Throughput Screening

Click-Chemistry Based Fluorometric Assay for Apolipoprotein N-acyltransferase from Enzyme Characterization to High-Throughput Screening

Published on: May 13, 2020

An Acetyl-Click Chemistry Assay to Measure Histone Acetyltransferase 1 Acetylation

An Acetyl-Click Chemistry Assay to Measure Histone Acetyltransferase 1 Acetylation

Published on: January 26, 2024

Area of Science:

Biochemistry
Hematology
Assay Development

Background:

ADAMTS-13 deficiency causes thrombotic thrombocytopenic purpura (TTP) via microvascular thrombi.
Existing ADAMTS-13 assays are complex and time-consuming.
A need exists for a rapid and sensitive assay for ADAMTS-13 activity.

Purpose of the Study:

To develop a rapid and sensitive assay for ADAMTS-13 activity in plasma.
To verify the specific cleavage site of ADAMTS-13 on the designed substrate.

Main Methods:

Constructed an enzyme-linked substrate with a core ADAMTS-13 peptide, HRP, and biotin.
Quantified ADAMTS-13 activity by measuring unadsorbed HRP after substrate cleavage and streptavidin-agarose removal.
Assessed inhibitory antibodies by measuring residual ADAMTS-13 activity after incubation with test plasma.

Main Results:

The assay determined plasma ADAMTS-13 activity within 60 minutes with 5.8% CV using 1 µL of plasma.
Amino acid sequencing confirmed cleavage at the Tyr1605-Met1606 bond.
TTP patient plasma showed <2% ADAMTS-13 activity, with antibody titers ranging from undetectable to 81 BU mL⁻¹.

Conclusions:

The HRP-linked substrate assay is rapid, sensitive, and reproducible.
This assay effectively determines ADAMTS-13 activity and inhibitory antibody levels in plasma.
The developed assay offers a significant improvement over existing methods for TTP diagnosis and management.