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Extracellular trehalose utilization by Saccharomyces cerevisiae
Arghya Basu1, Soma Bhattacharyya, Paramita Chaudhuri
1Biotechnology Division, Indian Institute of Chemical Biology, 4 Raja S. C. Mullick Road, Kolkata 700032, India.
Biochimica Et Biophysica Acta
|January 18, 2006
Summary
Yeast cells grown on trehalose experience a longer lag phase, which is shortened by adding extracellular trehalase. This suggests yeast utilizes extracellular trehalose for growth by hydrolyzing it into glucose.
Area of Science:
- Microbiology
- Biochemistry
- Yeast Physiology
Background:
- Trehalose is a disaccharide that serves as an energy reserve in yeast.
- Understanding trehalose metabolism is crucial for optimizing yeast fermentation processes.
Purpose of the Study:
- To investigate the growth patterns of Saccharomyces cerevisiae strains on trehalose compared to glucose.
- To elucidate the role of extracellular trehalase in trehalose utilization and yeast growth.
Main Methods:
- Culturing haploid yeast strains (MATalpha and MATa) in glucose and trehalose media.
- Monitoring growth curves, extracellular trehalase activity, trehalose, and glucose levels.
- Assessing alcohol production and the effect of exogenous trehalase addition.
Main Results:
- Yeast grown on trehalose exhibited an extended lag phase compared to glucose.
- Increased extracellular trehalase activity correlated with decreased trehalose and increased glucose levels, initiating log-phase growth.
- Extracellular trehalase activity was detected, facilitating trehalose hydrolysis into glucose for cellular utilization and subsequent alcohol fermentation.
Conclusions:
- Extracellular trehalase plays a key role in enabling yeast growth on trehalose by providing readily available glucose.
- The addition of extracellular trehalase can mitigate the extended lag phase observed when yeast is grown on trehalose.