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Updated: Aug 13, 2026

Sexual Transmission of American Trypanosomes from Males and Females to Naive Mates
Published on: January 27, 2019
[Observations on the use of TESA blot for the serological diagnosis of Chagas' disease]
Vicente Amato Neto1, Cláudia Regina De Marchi, Cláudio Santos Ferreira
1Laboratório de Investigação Médica-Parasitologia, Hospital das Clínicas, Faculdade de Medicina, Universidade de São Paulo, São Paulo, SP, Brazil. amatonet@usp.br
Abstract:
TESA blot was compared with indirect hemagglutination, indirect immunofluorescence and ELISA tests. In sera from 30 participants infected with Trypanosoma cruzi, and in 30 non infected the four techniques produced entirely equivalent results, all positive and all negative, respectively. In cases admitted to be inconclusive or in visceral leishmaniasis, frequent false positives were detected. However, TESA blot contributed with the least proportion of them.
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American Trypanosomiasis
Western Blotting
The technique begins with separating proteins from the sample using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), followed by protein transfer, immunoblotting, and finally, protein detection.

