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Updated: Aug 4, 2026

A Simple, Robust, and High Throughput Single Molecule Flow Stretching Assay Implementation for Studying Transport of Molecules Along DNA
Published on: October 1, 2017
Towards single-molecule DNA sequencing: assays with low nonspecific adsorption
Alexander Krieg1, Thomas Ruckstuhl, Stefan Seeger
1Wellcome Trust Centre for Human Genetics, University of Oxford, Headington, Oxford OX3 7BN, UK. alexander.krieg@well.ox.ac.uk
Abstract:
New DNA sequencing techniques are currently being developed using single-molecule fluorescence-based detection of enzymatic double-strand synthesis. Such application requires surface architectures on which single-stranded templates can be immobilized. A further important attribute is a very low tendency to attract fluorescently labeled bases nonspecifically. On this account, the adsorption behaviour of Cy5-dNTPs on a variety of surface coatings was studied by performing real-time measurements of the DNA synthesis using a supercritical angle fluorescence biosensor. It is demonstrated that polyacrylic acid coatings are an excellent choice to minimize the nonspecific binding of the bases.
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