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Two new anti-rat macrophage monoclonal antibodies
Abstract:
There are relatively few monoclonal antibodies (MAbs) to rat monocyte/macrophages available. We describe here 2 new such antibodies. The first, 109.2, recognizes most rat monocyte/macrophages and all polymorphs. The antigen recognized by this antibody is upregulated by 15 mins exposure to PMA (Phorbol myristate acetate) but down regulated by overnight exposure to LPS (lipopolysaccharide). It is probably an adhesion molecule and is likely to represent the rat equivalent of CD11b. The second antibody, 112.1, recognizes lysozyme in rat macrophages, particularly alveolar macrophages. In addition it also recognizes lysozyme in hen, rabbit and human macrophages. It also recognizes lysozyme in other tissues such as Paneth cells and proximal renal tubular cells.
Insights
Two novel monoclonal antibodies (MAbs) targeting rat macrophages were developed. Antibody 109.2 identifies a CD11b-like molecule, while 112.1 detects lysozyme in various species and tissues.
Area of Science:
- Immunology
- Cell Biology
Background:
- Limited availability of monoclonal antibodies (MAbs) for rat monocyte/macrophage research.
- Need for specific reagents to study these immune cells in rats.
Purpose of the Study:
- To develop and characterize two new MAbs for rat monocyte/macrophages.
- To identify the antigens recognized by these novel antibodies.
Main Methods:
- Production and characterization of monoclonal antibodies.
- Immunophenotyping of rat immune cells.
- Analysis of antigen expression under different stimulation conditions (PMA, LPS).
Main Results:
- Antibody 109.2 recognizes most rat monocytes/macrophages and polymorphs, identifying an antigen likely equivalent to CD11b, modulated by PMA and LPS.
- Antibody 112.1 detects lysozyme in rat macrophages (especially alveolar), as well as in macrophages and tissues of other species (hen, rabbit, human).
Conclusions:
- The new MAbs, 109.2 and 112.1, provide valuable tools for studying rat macrophages and related cells.
- Antibody 109.2 targets a potential rat CD11b homolog, aiding research into adhesion molecules.
- Antibody 112.1 offers cross-species utility for lysozyme detection in macrophages and other cell types.