Related Experiment Video
Updated: Aug 13, 2026
10:31
Real-time Live Imaging of T-cell Signaling Complex Formation
Published on: June 23, 2013
Arrays of transfected mammalian cells for high content screening microscopy
Holger Erfle1, Rainer Pepperkok
1Cell Biology and Cell Biophysics Program, European Molecular Biology Laboratory, Heidelberg, Germany.
Methods in Enzymology
|January 18, 2006
Abstract:
In this chapter we describe protocols for reverse transfection to generate mammalian cell arrays for systematic gene knock-downs by RNAi or knock-ins by ectopic cDNA expression. The method is suitable for high content screening microscopy at a high spatial and temporal resolution allowing even time-lapse analysis of hundreds of samples in parallel.
