Preparation and characterization of recombinant golgin tethers
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Tension Gauge Tether Probes for Quantifying Growth Factor Mediated Integrin Mechanics and Adhesion
Published on: February 11, 2022
Ayano Satoh1, Matthew Beard, Graham Warren
1Department of Cell Biology, Yale University School of Medicine, New Haven, Connecticut, USA.
Methods in Enzymology
|January 18, 2006
Abstract:
Golgin tethers are integral or peripheral Golgi proteins with predicted coiled-coil domains and many are known to interact directly with small GTPases of the Ypt/Rab or Arl families. Here we describe the preparation of recombinant golgins: GM130, p115 (and truncations thereof), the N-terminal fragment of giantin, CASP, and golgin-84.

