Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Bystander effects in unicellular organisms.

Linda C DeVeaux1, Lynn S Durtschi, Jonathan G Case

  • 1Idaho Accelerator Center, Campus Box 8263, Idaho State University, Pocatello, ID 83209, USA. develind@isu.edu

Mutation Research
|January 18, 2006
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

An inclusive Research Education Community (iREC) Model to Facilitate Undergraduate Science Education Reform.

Frontiers in education·2024
Same author

Models of Classroom Assessment for Course-Based Research Experiences.

Frontiers in education·2024
Same author

Desert diversity: genome sequence of <i>Gordonia rubripertincta</i> cluster DJ phage Mossy and cluster DV phage Erutan.

Microbiology resource announcements·2024
Same author

Complete genome sequence of <i>Cellulomonas</i> sp., strain ES6, a chromate-reducing bacterium isolated from chromium-contaminated subsurface sediment.

Microbiology resource announcements·2023
Same author

Genome Sequences of Gordonia rubripertincta Phages LilyPad and PokyPuppy.

Microbiology resource announcements·2022
Same author

Factors affecting use of ballistics gelatin in laboratory studies of bacterial contamination in projectile wounds.

Military Medical Research·2018

Radiation exposure triggers bystander effects in yeast cells, causing mutations in nearby non-irradiated cells. This response was observed in fission yeast (Schizosaccharomyces pombe) and other cellular stresses.

Area of Science:

  • Cell Biology
  • Radiation Biology
  • Genetics

Background:

  • Radiation-induced bystander effects are observed in mammalian cells.
  • These effects can be transmitted via medium to non-irradiated cells.
  • A need exists for simpler models to study these intercellular communication phenomena.

Purpose of the Study:

  • To develop and validate an assay for detecting radiation-induced bystander effects in the unicellular eukaryote, fission yeast (Schizosaccharomyces pombe).
  • To investigate the dose-response relationship and conditions required for observing bystander effects in yeast.
  • To explore if other cellular stresses induce similar bystander responses.

Main Methods:

  • Irradiation of fission yeast (Schizosaccharomyces pombe) with 16-18 MeV electrons.

Related Experiment Videos

  • Co-culturing irradiated and unirradiated yeast cells to assess bystander responses.
  • Measuring forward mutation frequency to 2-deoxy-d-glucose resistance in bystander cells.
  • Exposing cells to other stresses like heat, UV, and bleomycin.

    • Main Results:

    • A significant increase in forward mutation to 2-deoxy-d-glucose resistance was observed in unirradiated bystander cells when irradiated cells reached approximately 50% survival.
    • Higher radiation doses did not further increase the mutation rate in bystander cells.
    • A high ratio of irradiated to unirradiated cells was necessary to detect the bystander response.
    • Other cellular stresses (heat, UV, bleomycin) also induced detectable responses in bystander cells.

    Conclusions:

    • Fission yeast (Schizosaccharomyces pombe) serves as a viable model system for studying radiation-induced bystander effects.
    • The observed effects suggest intercellular signaling pathways are conserved across different organisms.
    • The findings have implications for understanding radiation biology and potentially evolutionary mechanisms of cellular communication.