Related Experiment Video
Updated: Aug 13, 2026

Free Radicals in Chemical Biology: from Chemical Behavior to Biomarker Development
Published on: April 15, 2013
Flow injection determination of adenine at trace level based on luminol-K2Cr2O7 chemiluminescence in a micellar
1College of Chemistry and Life Sciences, Tianjin Normal University, Tianjin 300074, PR China. liueb123@163.com
Abstract:
A novel flow injection chemiluminescence (CL) analysis method for the determination of adenine in the presence of sodium dodecylbenzene sulfonate (SDBS) surfactant micelles is described. This method is based on the luminescent properties of the luminol-K2Cr2O7-adenine in alkaline medium sensitized by SDBS. The optimized experimental conditions were evaluated and the possible mechanism was discussed by examining CL emission spectrum and the effect of various free radical scavengers on CL emission intensity. The CL increase is linearly related to the concentration of adenine in the range of 2.92x10(-6) to 4.38x10(-10) mol l-1 with a detection limit of 2.46x10(-10) mol l-1 (S/N=3). The relative standard deviation for 2.92x10(-7) mol l-1 samples was 1.67% (n=12). The proposed method has been applied to the determination of adenine in human serum.
Related Concept Videos
Photoluminescence: Applications
Flame Photometry: Lab
Atomic Absorption Spectroscopy: Lab
Solutions containing organic solvents, such as low-molecular-mass alcohols, esters, or ketones, enhance absorbances by increasing nebulizer...

