Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Studying plant-microbe interactions using stable isotope technologies.

Jim I Prosser1, J Ignacio Rangel-Castro, Ken Killham

  • 1School of Biological Sciences, University of Aberdeen, St Machar Drive, Aberdeen AB24 3UU, United Kingdom. j.prosser@abdn.ac.uk

Current Opinion in Biotechnology
|January 18, 2006
PubMed
Summary

Stable isotope probing offers a cultivation-independent method to study plant-microorganism interactions in the rhizosphere. This technique reveals active microbial communities utilizing plant root exudates, advancing our understanding beyond traditional methods.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Degradation of the polycyclic aromatic hydrocarbon (PAH) fluorene is retarded in a Scots pine ectomycorrhizosphere.

The New phytologist·2021
Same author

Effect of arsenic contaminated irrigation water on Lens culinaris L. and toxicity assessment using lux marked biosensor.

Journal of environmental sciences (China)·2013
Same author

A 13C-NMR study of exudation and storage of carbohydrates and amino acids in the ectomycorrhizal edible mushroom Cantharellus cibarius.

Mycologia·2010
Same author

Factors affecting benthic impacts at Scottish fish farms.

Environmental science & technology·2010
Same author

Mycorrhizas and biomass crops: opportunities for future sustainable development.

Trends in plant science·2009
Same author

A model for bacterial conjugal gene transfer on solid surfaces.

FEMS microbiology ecology·2009

Area of Science:

  • Microbial Ecology
  • Plant-Microbe Interactions
  • Environmental Science

Background:

  • Rhizosphere interactions between plants and microorganisms are intricate, involving nutrient exchange and molecular signaling.
  • Traditional cultivation-based methods limit the comprehensive study of these complex soil ecosystems.
  • Root exudates are key mediators of plant-microbe communication and nutrient cycling.

Purpose of the Study:

  • To highlight the potential of stable isotope probing (SIP) for studying rhizosphere microbial communities.
  • To emphasize SIP as a cultivation-independent technique for tracking carbon assimilation from root exudates.
  • To explore the application of SIP for mechanistic studies of specific plant-microbe interactions.

Main Methods:

  • Stable isotope probing (SIP) to trace carbon flow from plant root exudates to soil microorganisms.

Related Experiment Videos

  • Cultivation-independent analysis of microbial communities actively assimilating labeled carbon.
  • Application of SIP for characterizing organisms involved in rhizosphere carbon cycling.

    • Main Results:

    • SIP enables the identification of microorganisms actively utilizing plant-derived carbon in the soil.
    • Current SIP applications primarily focus on low-specificity interactions.
    • The technique shows promise for investigating specific plant-microbe signaling pathways.

    Conclusions:

    • Stable isotope probing is a powerful tool for understanding rhizosphere microbial ecology.
    • Further development is needed to increase SIP sensitivity for detailed mechanistic studies.
    • SIP facilitates a deeper understanding of plant-microorganism dynamics without cultivation.